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Ever, we have observed that suboptimal sample collection as determined by ERV3 detection and presence of visible mould in swabsamples reaching the laboratory can negatively affect sample quality and potentially respiratory virus detection. The data from the first 20-months of our longitudinal study indicate that respiratory virus detection is associated with the ERV3 load in nasal swab specimens.
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Y arrival should be monitored and feedback provided if delays occur. Finally, if delays are expected swabs should be placed in the household refrigerator until mailed to the laboratory [20].Abbreviations ARI: Acute respiratory infection; Ct: Cycle threshold; DNA: Deoxyribonucleic acid; EHV1: Equine herpes virus 1; ERV3: Endogenous retrovirus 3; ITS: Internal transcribed spacer; OR: Odds ratio; ORC
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Was especially evident during the warm, humid spring and summer months, which leads us to speculate that fungal contamination occurred during sample collection and was influenced by the aforementioned environmental factors. Unfortunately, we could not explore this further as it was beyond the scope of the present study. In addition, while mould growth proved to be an issue in the subtropical clima
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Ar punctate GFP signals corresponding to the typical Golgi localization pattern (Gao et al., 2012, 2014a). However, strong vacuolar GFP signals were detected after knockdown of -COP by DEX induction, suggesting mislocalization of AtEMP12-GFP-RNIKCD to the vacuole (Figure 7B). These results are consistent with previous transient expression results showing mislocalization of AtEMP12-GFP to post-Golg
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